Quimica nova | |
Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection | |
Ferreira, Thais P.1  Magalhães, Viviane S.1  Scott, Fábio B.1  Silva, Nathália C. C.1  Medeiros, Deborah M. V. C.1  Cid, Yara P.1  Oliveira, Rodrigo M.1  | |
[1] Universidade Federal Rural do Rio de Janeiro, Seropédica, Brasil | |
关键词: fipronil; bovine plasma; LC-UV.; | |
DOI : 10.1590/S0100-40422012001000029 | |
学科分类:化学(综合) | |
来源: Sociedade Brasileira de Quimica | |
【 摘 要 】
A fast and efficientmethod has been developed and validated for the determination of fipronil inbovine plasma. Samples were subjected to solid-phase extraction (SPE) followedby reversed phase liquid chromatography (LC) separation, using acetonitrile/water(60:40 v/v) as the mobile phase with a flow rate of 1.0 mL/min and ultraviolet(UV) detection at 210 nm. Ethiprole was used as the internal standard (IS).The method was found to be linear over the range 5-500 ng/mL (r = 0.999). Thelimit of quantitation (LOQ) was validated at 5 ng/mL. The method was successfullyapplied to monitor plasma concentrations following subcutaneous administrationof fipronil in cattle.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO201902019415039ZK.pdf | 351KB | download |