Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection

Quimica nova

Ferreira, Thais P.¹  Magalhães, Viviane S.¹  Scott, Fábio B.¹  Silva, Nathália C. C.¹  Medeiros, Deborah M. V. C.¹  Cid, Yara P.¹  Oliveira, Rodrigo M.¹ 

[1] Universidade Federal Rural do Rio de Janeiro, Seropédica, Brasil

关键词: fipronil;    bovine plasma;    LC-UV.;   

DOI  :  10.1590/S0100-40422012001000029

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A fast and efficientmethod has been developed and validated for the determination of fipronil inbovine plasma. Samples were subjected to solid-phase extraction (SPE) followedby reversed phase liquid chromatography (LC) separation, using acetonitrile/water(60:40 v/v) as the mobile phase with a flow rate of 1.0 mL/min and ultraviolet(UV) detection at 210 nm. Ethiprole was used as the internal standard (IS).The method was found to be linear over the range 5-500 ng/mL (r = 0.999). Thelimit of quantitation (LOQ) was validated at 5 ng/mL. The method was successfullyapplied to monitor plasma concentrations following subcutaneous administrationof fipronil in cattle.

【 授权许可】

CC BY