Ameliorative effects of sea buckthorn oil on DNCB induced atopic dermatitis model mice via regulation the balance of Th1/Th2

BMC Complementary Medicine and Therapies

Diandong Hou¹  Xiaowei Han¹  Ping Lei¹  Ming Xu¹  Hongquan Guan¹  Dongning Kong²  Xinxin Wang³  Sijia Li³  Jiping Liu⁴ 

[1] College of Integrated Traditional Chinese and Western Medicine, Liaoning University of Traditional Chinese Medicine;Liaoning Dongning Pharmceutical Co., Ltd.;Liaoning University of Traditional Chinese Medicine;Neurosurgery Department, Northern Hospital of Inner Mongolia;

关键词: Atopic dermatitis;    Sea buckthorn oil;    2,4-dinitrochlorobenzene;    Cytokine;   

DOI  :  10.1186/s12906-020-02997-2

来源: DOAJ

【 摘 要 】

Abstract Background Atopic dermatitis (AD) is a worldwide chronic skin disease which burden public health. Sea buckthorn (SBT) (Hippophae rhamnoides L., Elaeagnaceae) oil, as a traditional herbal medicine, has been used for disease treatment for many years. The effects of SBT oil on AD mouse model induced by repeated administration of 2,4-dinitrochlorobenzene (DNCB) in BALB/c mice was evaluated in this study. Methods Mice were divided into four groups including the normal control group, AD model group, AD model group treated with SBT oil (5 ml/kg) and AD model group treated with SBT oil (10 ml/kg). Same volume at different concentrations of SBT oil was applied daily on the latter two groups by gavage for 15 days following AD model induction. The function of skin barrier and the production of IL-4, IFN-γ, TNF-α and TSLP were examined after animal sacrifice. The migration and mature of langerhans cell (LCs) in lymph node was further assessed by flow cytometry. Results SBT oil alleviated dermatitis scores, decreased ear thickness, prevented infiltration of mast cell, reduced lymph node weight and depressed activity of Th2 cells. SBT oil also reduced the expression of IL-4, IFN-γ, TNF-α and TSLP in ear tissue, IgE level in serum and mRNA relative expression of IL-4, IFN-γ, TNF-α in lymph node. Moreover, SBT oil inhibited the migration of LCs cells from local lesions to lymph node and it’s mature in lymph node. Conclusions These results suggest SBT oil had a beneficial effect either systemic or regional on DNCB-induced AD mice via maintain the balance of Th1/Th2 and may be a potential complementary candidate for AD treatment.

【 授权许可】

Unknown