Molecular Epidemiology of Hypervirulent Carbapenemase-Producing Klebsiella pneumoniae

Frontiers in Cellular and Infection Microbiology

Wenjie Chen¹  Yuming Li²  Dongxing Tian³  Xiaofei Jiang³  Weiwen Wang³  Dakang Hu³  Pan Fu⁴  Ping Ren⁵  Xiaobin Li⁶ 

[1] Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China;Department of Intensive Care Unit, Huashan Hospital, Fudan University, Shanghai, China;Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, China;Department of Microbiology, Children’s Hospital of Fudan University, Shanghai, China;Zhejiang Provincial Demonstration Center of Laboratory Medicine Experimental Teaching, Wenzhou Medical University, Wenzhou, China;Zhuhai Precision Medical Center, Zhuhai People’s Hospital (Zhuhai Hospital Affiliated With Jinan University), Zhuhai, China;

关键词: Klebsiella pneumoniae;    virulence;    plasmid;    blaKPC;    epidemiology;   

DOI  :  10.3389/fcimb.2021.661218

来源: DOAJ

【 摘 要 】

ObjectiveTo investigate the overall distributions of key virulence genes in Klebsiella pneumoniae, especially the hypervirulent blaKPC-positive K. pneumoniae (Hv-blaKPC(+)-KP).MethodsA total of 521 complete genomes of K. pneumoniae from GenBank were collected and analyzed. Multilocus sequence typing, molecular serotyping, antibiotic-resistance, virulence genes and plasmid replicon typing were investigated.ResultsPositive rates of virulence genes highly varied, ranging from 2.9 (c-rmpA/A2) to 99.6% (entB). Totally 207 strains presented positive fimH, mrkD, entB and wzi and 190 showed positive fimH, mrkD, entB, irp2 and wzi, which were the two primary modes. A total of 94, 165 and 29 strains were denoted as hypervirulent K. pneumoniae (HvKP), blaKPC(+)-KP and Hv-blaKPC(+)-KP. ST11 accounted for 17 among the 29 Hv-blaKPC(+)-KP strains; Genes iucA, p-rmpA2 and p-rmpA were positive in 28, 26 and 18 Hv-blaKPC(+)-KP strains respectively. Among the 29 Hv-blaKPC(+)-KP strains exhibiting four super clusters from GenBank, IncHI1B plasmids carrying virulence genes and IncFII ones with blaKPC were responsible for both 23 strains respectively.ConclusionsPositive rates of virulence genes vary remarkably in K. pneumoniae. Genes iucA, p-rmpA2 and p-rmpA were primary ones inducing Hv-blaKPC(+)-KP. IncHI1B plasmids carrying virulence genes and IncFII ones with blaKPC constitute the primary combination responsible for Hv-blaKPC(+)-KP. The making of Hv-blaKPC(+)-KP is mostly via blaKPC(+)-KP acquiring another plasmid harboring virulence genes.

【 授权许可】

Unknown