Cytosolic and Nucleosolic Calcium-Regulated Molecular Networks in Response to Long-Term Treatment with Abscisic Acid and Methyl Jasmonate in Arabidopsis thaliana

Genes

Yanli Nie¹  Feifei Huang¹  Jin Luo¹  Shengcheng Han¹  Doudou Wang¹  Yingdian Wang¹  Heping Zhao¹  Lvli Chen¹  Pengcheng Yan² 

[1] Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China;Department of Computational Biology, Beijing Computing Center, Beijing 100094, China;

关键词: cytosolic calcium-regulated hormone-responsive gene;    nucleosolic calcium-regulated hormone-responsive gene;    gene co-expression network;    hub gene;    A. thaliana;   

DOI  :  10.3390/genes13030524

来源: DOAJ

【 摘 要 】

Calcium acts as a universal secondary messenger that transfers developmental cues and stress signals for gene expression and adaptive growth. A prior study showed that abiotic stresses induce mutually independent cytosolic Ca²⁺ ([Ca²⁺]_cyt) and nucleosolic Ca²⁺ ([Ca²⁺]_nuc) increases in Arabidopsis thaliana root cells. However, gene expression networks deciphering [Ca²⁺]_cyt and [Ca²⁺]_nuc signalling pathways remain elusive. Here, using transgenic A. thaliana to selectively impair abscisic acid (ABA)- or methyl jasmonate (MeJA)-induced [Ca²⁺]_cyt and [Ca²⁺]_nuc increases, we identified [Ca²⁺]_cyt- and [Ca²⁺]_nuc-regulated ABA- or MeJA-responsive genes with a genome oligo-array. Gene co-expression network analysis revealed four Ca²⁺ signal-decoding genes, CAM1, CIPK8, GAD1, and CPN20, as hub genes co-expressed with Ca²⁺-regulated hormone-responsive genes and hormone signalling genes. Luciferase complementation imaging assays showed interactions among CAM1, CIPK8, and GAD1; they also showed interactions with several proteins encoded by Ca²⁺-regulated hormone-responsive genes. Furthermore, CAM1 and CIPK8 were required for MeJA-induced stomatal closure; they were associated with ABA-inhibited seed germination. Quantitative reverse transcription polymerase chain reaction analysis showed the unique expression pattern of [Ca²⁺]-regulated hormone-responsive genes in cam1, cipk8, and gad1. This comprehensive understanding of distinct Ca²⁺ and hormonal signalling will allow the application of approaches to uncover novel molecular foundations for responses to developmental and stress signals in plants.

【 授权许可】

Unknown