Integrated time-serial transcriptome networks reveal common innate and tissue-specific adaptive immune responses to PRRSV infection

Veterinary Research

Tae-Hun Kim¹  Kyung-Tai Lee¹  Haesu Gho¹  Choi-Kyu Park²  Sang-Myeong Lee³  Won-Il Kim⁴  Chang-Gi Jeong⁴  Seung-Chai Kim⁴  Byeonghwi Lim⁵  Jun-Mo Kim⁵  Sangwook Kim⁵  Kyu-Sang Lim⁶  Marinus F. W. te Pas⁷ 

[1] Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA;College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University;College of Veterinary Medicine, Chungbuk National University;College of Veterinary Medicine, Jeonbuk National University;Department of Animal Science and Technology, Chung-Ang University;Department of Animal Science, Iowa State University;Wageningen UR Livestock Research;

关键词: adaptive immunity;    gene co-expression network;    innate immunity;    integrated transcriptomes;    porcine reproductive and respiratory syndrome virus;   

DOI  :  10.1186/s13567-020-00850-5

来源: DOAJ

【 摘 要 】

Abstract Porcine reproductive and respiratory syndrome virus (PRRSV) infection is the most important viral disease causing severe economic losses in the swine industry. However, mechanisms underlying gene expression control in immunity-responsible tissues at different time points during PRRSV infection are poorly understood. We constructed an integrated gene co-expression network and identified tissue- and time-dependent biological mechanisms of PRRSV infection through bioinformatics analysis using three tissues (lungs, bronchial lymph nodes [BLNs], and tonsils) via RNA-Seq. Three groups with specific expression patterns (i.e., the 3-dpi, lung, and BLN groups) were discovered. The 3 dpi-specific group showed antiviral and innate-immune signalling similar to the case for influenza A infection. Moreover, we observed adaptive immune responses in the lung-specific group based on various cytokines, while the BLN-specific group showed down-regulated AMPK signalling related to viral replication. Our study may provide comprehensive insights into PRRSV infection, as well as useful information for vaccine development.

【 授权许可】

Unknown