Optimization of oxalic acid pre-treatment and enzymatic saccharification in Typha latifolia for production of reducing sugar

Journal of Genetic Engineering and Biotechnology

Sunil Kodishetty Ramaiah¹  Girisha Shringala Thimappa¹  Lokesh Kyathasandra Nataraj²  Proteek Dasgupta³ 

[1] Bioenergy Lab, Department of Biotechnology, Bangalore University;Department of Biotechnology, M.S. Ramaiah Institute of Technology;Department of Zoology, Bangalore University;

关键词: Optimization;    Oxalic acid;    Pre-treatment;    Reducing sugar;    Saccharification;    Typha latifolia;   

DOI  :  10.1186/s43141-020-00042-w

来源: DOAJ

【 摘 要 】

Abstract Background Plants with high biomass can be manipulated for their reducing sugar content which ultimately upon fermentation produces ethanol. This concept was used to enhance the production of reducing sugar from cattail (Typha latifolia) by oxalic acid (OAA) pre-treatment followed by enzymatic saccharification. Result The optimum condition of total reducing sugar released from OAA pre-treatment was found to be 22.32 mg/ml (OAA—1.2%; substrate concentration (SC)—6%; reaction time (RT)—20 min) using one variable at a time (OVAT). Enzymatic saccharification yielded 45.21 mg/ml of reducing sugar (substrate concentration (SC)—2.4%; enzymatic dosage—50 IU/g; pH 7.0; temp—50 °C) using response surface methodology (RSM). Conclusion We conclude that Typha can be used as a potential substrate for large-scale biofuel production, employing economical bioprocessing strategies.

【 授权许可】

Unknown