SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH

Journal of The Chilean Chemical Society

SILVA, PAULO A. B. DA¹  SOUZA, GUSTAVO C. S. DE² 

[1] Instituto Federal de Alagoas, Maceió, Brasil;Universidade Federal de Pernambuco, Recife, Brasil

关键词: Dialysis;    Flow injection analysis;    Multicommutation;    Reducing sugar;    Wine.;   

DOI  :  10.4067/s0717-97072018000203994

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

A multicommuted flow procedure for the spectrophotometric determination of reducing sugars in wine has been developed. A dialysis unit was incorporated in the system to minimize matrix interferences and eliminate the colored compounds of the wine. The oxidation-reduction reaction in an alkaline medium between reducing sugars and the Cu2+-Neocuproine complex was employed. The compound formed was monitored at 460 nm. In the proposed procedure, there was a linear response between 0.25 and 4.0 g L-1 reducing sugars (y = (0.36 ± 0.02) + (0.31 ± 0.01) * x, R = 0.9975, n = 6), a coefficient of variance of 2.6% for a reducing sugar solution of 2.0 g L-1 (n = 7), a limit of detection estimated at 0.03 g L-1, reagent consumption per determination of 0.330, 0.128 and 0.160 mg for neocuproine, CuSO4 and NaOH, respectively. A sampling throughput of 45 determinations per hour was achieved. The proposed system was applied to wine samples and comparing the results with an AOAC official method no significant difference at a 95% confidence level.

【 授权许可】

Unknown