CD4+ T Cell Immune Specificity Changes After Vaccination in Healthy And COVID-19 Convalescent Subjects

Frontiers in Immunology

Francisco Sanchez-Madrid¹  Arantzazu Alfranca¹  Hugh T. Reyburn²  José M. Rodríguez Frade²  Mar Valés-Gómez²  José M. Casasnovas³  Enrique Martín-Gayo⁴  Cecilia Muñoz-Calleja⁴  Carlos Vilches⁵  Pedro Martínez-Fleta⁶  Laura Esparcia-Pinedo⁶  Paula Vera-Tomé⁶  Noelia Ropero⁶ 

[1] Cardiovascular Centre for Biomedical Research Network (CIBER CV) Health Institute Carlos III, Madrid, Spain;Department of Immunology and Oncology, Centro Nacional de Biotecnologia/Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain;Department of Macromolecular Structures, Centro Nacional de Biotecnologia/Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain;Department of Medicine, School of Medicine, Universidad Autónoma of Madrid, Madrid, Spain;Immunogenetics and Histocompatibility, Instituto de Investigación Sanitaria Puerta de Hierro-Segovia de Arana, Madrid, Spain;Immunology Department, Hospital Universitario de La Princesa, Instituto de Investigación Sanitaria Princesa (IIS-IP), Madrid, Spain;

关键词: T cell;    adaptive immunity;    COVID-19;    vaccination;    immune profile;   

DOI  :  10.3389/fimmu.2021.755891

来源: DOAJ

【 摘 要 】

The immune response promoted by SARS-CoV-2 vaccination is relevant to develop novel vaccines and optimized prevention strategies. We analyzed the adaptive immunity in healthy donors (HD) and convalescent individuals (CD), before and after administering BNT162b2 vaccine. Our results revealed specific changes in CD4+ T cell reactivity profile in vaccinated HD and CD, with an increase in S1 and S2 positive individuals, proportionally higher for S2. On the contrary, NCAP reactivity observed in HD and CD patients was no longer detectable after vaccination. Despite the substantial antibody response in CD, MPro-derived peptides did not elicit CD4+ lymphocyte activation in our assay in either condition. HD presented an increment in anti-S and anti-RBD IgG after first dose vaccination, which increased after the second vaccination. Conversely, anti-S and anti-RBD IgG and IgA titers increased in already positive CD after first dose administration, remaining stable after second dose inoculation. Interestingly, we found a strong significant correlation between S1-induced CD4+ response and anti-S IgA pre-vaccination, which was lost after vaccine administration.

【 授权许可】

Unknown