Sourcing Antibiotic-Resistant Escherichia coli in Aquatic Ecosystems: A Combined Laboratory and Field Module

Journal of Microbiology & Biology Education

Matthew J. Heard¹  Christopher E. Barton¹  Victoria J. Frost²  Rachel Hongo³ 

[1] Department of Biology, Belmont University, Nashville, Tennessee, USA;Department of Biology, Winthrop University, Rock Hill, South Carolina, USA;Graduate Program in Biomedical and Biological Sciences, Harvard Medical School, Boston, Massachusetts, USA;

关键词: Escherichia coli;    antibiotics;    environmental;    pathogen;    phylogroup;    water quality;   

DOI  :  10.1128/jmbe.00207-21

来源: DOAJ

【 摘 要 】

ABSTRACT The emergence of antibiotic-resistant bacteria represents a growing threat in aquatic ecosystems. In this combined field and laboratory activity, students will determine whether Escherichia coli, an indicator bacteria species commonly found in aquatic ecosystems, shows signs of resistance to common antibiotics. In addition, students will use molecular biology techniques to identify whether Escherichia coli cells sourced from different hosts (i.e., phylogroups) show different patterns of antibiotic resistance. This activity will help students to gain experience in environmental microbiology, environmental science, molecular biology, and public health. This module is also designed to provide instructors with flexibility to pick and choose activities that best meet the needs of their class or research program.

【 授权许可】

Unknown