Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification

BioTechniques

Francisca Serra¹  Andreu Palou²  Bàrbara Reynés² 

[1] Biotechnology, NUO Group, Universitat de les Illes Balears, Palma, 07122, Spain;;1Laboratory of Molecular Biology, Nutrition &

关键词: COVID-19;    nucleocapsid primers;    ORF1ab primers;    SARS-CoV-2 detection;    virus LAMP amplification;   

DOI  :  10.2144/btn-2020-0159

来源: DOAJ

【 摘 要 】

Evaluation of the performance of a new set of primers defined from the ORF1ab sequence, and its combination with a previously published set of primers from the N sequence, to detect SARS-CoV-2 RNA by the loop-mediated isothermal amplification technique is presented. The ORF1ab primer set enables visual detection of SARS-CoV-2 RNA in 16 min. In addition, a simultaneous reaction with both ORF1ab and N primers allows for higher sensitivity of detection, particularly when low numbers of copies are present (250 viral RNA copies). Further, the protocol is able to detect viral RNA in saliva samples. The procedure reported could be easily implemented in the generation of a new and sensitive rapid point-of care device for SARS-CoV-2 RNA visual detection.

【 授权许可】

Unknown