Hydrogel-Coated Nanonet-Based Field-Effect Transistors for SARS-CoV-2 Spike Protein Detection in High Ionic Strength Samples

Engineering Proceedings

article

Alexandra Parichenko¹  Wonyeong Choi²  Seonghwan Shin²  Marlena Stadtmüller³  Teuku Fawzul Akbar⁴  Carsten Werner⁴  Jeong-Soo Lee²  Bergoi Ibarlucea¹  Gianaurelio Cuniberti¹ 

[1] Institute for Materials Science and Max Bergmann Center for Biomaterials, Dresden University of Technology;Department of Electrical Engineering, Pohang University of Science and Technology;Universitätsklinikum Carl Gustav Carus Dresden;Max Bergmann Center for Biomaterials, Leibniz Institute of Polymer Research Dresden

关键词: COVID-9 diagnostics;    SARS-CoV-2 detection;    hydrogel biosensor;    field-effect transistor;    Debye screening length;    starPEG;   

DOI  :  10.3390/IECB2023-14566

来源: mdpi

【 摘 要 】

The SARS-CoV-2 pandemic has triggered many studies worldwide in the area of biosensors, leading to innovative approaches for the quantitative assessment of COVID-19. A nanostructured field-effect transistor (FET) is one type of device shown to be ultrasensitive for virus determination. FETs can be used as transducers to analyze changes in electrical current caused by the bonding of viral molecules to the surface of the semiconducting nanomaterial layer of the FETs. Although nano-transistors require simple setups amenable to be miniaturized for point-of-care diagnostic of COVID-19, this type of sensor usually has limited sensitivity in biological fluids. The reason behind this is the shortened screening length in the presence of high ionic strength solutions. In the frame of this study, we propose a methodology consisting of the FET surface modification with a hydrogel based on the star-shaped polyethylene glycol (starPEG), which hosts specific antibodies against SARS-CoV-2 spike protein in its porous structure. The deposition of the hydrogel increases the effective Debye length, preserving the biosensor’s sensitivity. We demonstrate the capability of silicon nanonet-based FETs to detect viral antigens and cultured viral particles in phosphate-buffered saline (PBS) as well as in human-purified saliva. Finally, we discriminated between positive and negative patients’ nasopharyngeal swab samples.

【 授权许可】

Unknown