Inhibitory Effect of Camptothecin against Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae RS-2

Molecules

Ju Luo¹  Bin Li¹  Qiaolin Dong²  Syed Ishtiaq Anjum²  Guochang Sun²  Wen Qiu³  Li Cai⁴  Guanlin Xie⁴  Mingsheng Hou⁴ 

[1] Technology, Huazhong Agricultural University, Wuhan 430070, China;;College of Plant Science &State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China;

关键词: antibacterial activity;    antibacterial mechanism;    CPT;    Hcp;    gene expression;   

DOI  :  10.3390/molecules21080978

来源: DOAJ

【 摘 要 】

Camptothecin (CPT) has anticancer, antiviral, and antifungal properties. However, there is a dearth of information about antibacterial activity of CPT. Therefore, in this study, we investigated the inhibitory effect of CPT on Acidovorax avenae subsp. avenae strain RS-2, the pathogen of rice bacterial brown stripe, by measuring cell growth, DNA damage, cell membrane integrity, the expression of secretion systems, and topoisomerase-related genes, as well as the secretion of effector protein Hcp. Results indicated that CPT solutions at 0.05, 0.25, and 0.50 mg/mL inhibited the growth of strain RS-2 in vitro, while the inhibitory efficiency increased with an increase in CPT concentration, pH, and incubation time. Furthermore, CPT treatment affected bacterial growth and replication by causing membrane damage, which was evidenced by transmission electron microscopic observation and live/dead cell staining. In addition, quantitative real-time PCR analysis indicated that CPT treatment caused differential expression of eight secretion system-related genes and one topoisomerase-related gene, while the up-regulated expression of hcp could be justified by the increased secretion of Hcp based on the ELISA test. Overall, this study indicated that CPT has the potential to control the bacterial brown stripe pathogen of rice.

【 授权许可】

Unknown