Antibacterial Activity of Chinese Red Propolis against Staphylococcus aureus and MRSA

Molecules

Di Wu¹  Kai Wang²  Sha Yan²  Suzhen Qi²  Xiaofeng Xue²  Liming Wu²  Wenwen Zhang²  Gomez Escalada Margarita³  Wenqin Yuan⁴ 

[1] College of Animal Science, Shanxi Agricultrual University, Jinzhong 030801, China;Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100094, China;School of Health, Leeds Beckett University, Leeds LS1 3HE, UK;School of Life Science, Liaocheng University, Liaocheng 252000, China;

关键词: Chinese red propolis;    antibacterial activity;    metabolomics;    antibacterial mechanism;   

DOI  :  10.3390/molecules27051693

来源: DOAJ

【 摘 要 】

The antibacterial activity of propolis has long been of great interest, and the chemical composition of propolis is directly dependent on its source. We recently obtained a type of propolis from China with a red color. Firstly, the antibacterial properties of this unusual propolis were determined against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). Studies on its composition identified and quantified 14 main polyphenols of Chinese red propolis extracts (RPE); quantification was carried out using liquid chromatography triple quadrupole tandem mass spectrometry (LC-QQQ-MS/MS) and RPE was found to be rich in pinobanksin, pinobanksin-3-acetate, and chrysin. In vitro investigations of its antibacterial activity revealed that its activity against S. aureus and MRSA is due to disruption of the cell wall and cell membrane, which then inhibits bacterial growth. Despite its similar antibacterial activities against S. aureus and MRSA, metabolomic analysis further revealed the effects of RPE on bacteria metabolism were different. The untargeted metabolomic results showed that a total of 7 metabolites in 12 metabolic pathways had significant changes (Fold change > 2, p < 0.05 *) after RPE treatment in S. aureus, while 11 metabolites in 9 metabolic pathways had significant changes (Fold change > 2, p < 0.05 *) after RPE treated on MRSA. Furthermore, RPE downregulated several specific genes related to bacterial biofilm formation, autolysis, cell wall synthesis, and bacterial virulence in MRSA. In conclusion, the data obtained indicate that RPE may be a promising therapeutic agent against S. aureus and MRSA.

【 授权许可】

Unknown