Enriching for human acute myeloid leukemia stem cells using reactive oxygen species-based cell sorting

STAR Protocols

Cristiana O’Brien¹  Brett M. Stevens²  Courtney L. Jones²  Craig T. Jordan³ 

[1] Corresponding author;Division of Hematology, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA;Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada;

关键词: Flow cytometry/mass cytometry;    Cancer;    Metabolism;    Stem cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Isolation of leukemia stem cells presents a challenge due to the heterogeneity of the immunophenotypic markers commonly used to identify blood stem cells. Several studies have reported that relative levels of reactive oxygen species (ROS) can be used to enrich for stem cell populations, suggesting a potential alternative to surface antigen-based methods. Here, we describe a protocol to enrich for stem cells from human acute myeloid leukemia specimens using relative levels of ROS. This protocol provides consistent enrichment of leukemia stem cells.For complete details on the use and execution of this protocol, please refer to Lagadinou et al. (2013) and Pei et al. (2018).

【 授权许可】

Unknown