Horizontal mtDNA transfer between cells is common during mouse development

iScience

Crystal Van Dyken¹  Eunju Kang¹  Aleksei Mikhalchenko¹  Tomonari Hayama¹  David Yoon¹  Shoukhrat Mitalipov¹  Amy Koski¹  Anthony Paul Barnes¹  Dan Liang²  Ying Li²  Paula Amato²  Nuria Marti Gutierrez²  David Battaglia²  Rebecca Tippner-Hedges²  Hong Ma²  Yeonmi Lee² 

[1] Science University, Portland, OR 97239, USA;;Center for Embryonic Cell and Gene Therapy, Oregon Health &

关键词: Molecular biology;    Cell biology;    Developmental biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Cells transmit their genomes vertically to daughter cells during cell divisions. Here, we demonstrate the occurrence and extent of horizontal mitochondrial (mt)DNA acquisition between cells that are not in a parent-offspring relationship. Extensive single-cell sequencing from various tissues and organs of adult chimeric mice composed of cells carrying distinct mtDNA haplotypes showed that a substantial fraction of individual cardiomyocytes, neurons, glia, intestinal, and spleen cells captured donor mtDNA at high levels. In addition, chimeras composed of cells with wild-type and mutant mtDNA exhibited increased trafficking of wild-type mtDNA to mutant cells, suggesting that horizontal mtDNA transfer may be a compensatory mechanism to restore compromised mitochondrial function. These findings establish the groundwork for further investigations to identify mtDNA donor cells and mechanisms of transfer that could be critical to the development of novel gene therapies.

【 授权许可】

Unknown