Chemotactic behavior of egg mitochondria in response to sperm fusion in mice

Heliyon

Woojin Kang¹  Keiichi Yoshida²  Yoshitaka Miyamoto³  Yuichirou Harada⁴  Toshio Hamatani⁴  Kenji Miyado⁵  Rinako Miyabayashi⁶  Mamoru Tanaka⁷  Mitsutoshi Yamada⁷  Akihiro Umezawa⁷  Maki Iwai⁷  Hidekazu Saito⁸  Natsuko Kawano⁸  Mami Miyado⁸  Akihiro Nakamura⁹ 

[1] Corresponding author.;Department of Life Sciences, School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama, Kawasaki, Kanagawa 214-8571, Japan;Department of Perinatal Medicine and Oocyte Care, National Center for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan;Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan;Department of Molecular Endocrinology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan;Department of Molecular Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku, Tokyo 192-0397, Japan;Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan;Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan;Shinbashi YUME Clinic, 2-5-1 Shinbashi, Miyato, Tokyo 105-0004, Japan;

关键词: Cell biology;    Developmental biology;    Molecular biology;    Zoology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca2+ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca2+ storage and were colocalized with endoplasmic reticulum (ER) during Ca2+ oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca2+ oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca2+ oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation.

【 授权许可】

Unknown