A metabolic labeling protocol to enrich myristoylated proteins from Caenorhabditis elegans

STAR Protocols

Yanwen Feng¹  Hongyun Tang²  Xin Gong³ 

[1] Institute of Biology, Westlake Institute for Advanced Study, Hangzhou 310024, China;Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, China;Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou 310024, China;

关键词: Metabolism;    Model Organisms;    Molecular Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Myristoylation is a type of lipidation with important functions. Owing to the lack of high-quality antibodies against myristoylation, developing alternative methods for profiling myristoylated proteins is important. Here, we provide a protocol for metabolic labeling using click chemistry to profile myristoylated proteins in C. elegans. Our approach improves the signal/noise ratio by covalently linking the myristoylated proteins to the beads. This protocol provides a highly specific and reproducible way for enriching myristoylated proteins, which could be modified to analyze other types of lipidations.For complete details on the use and execution of this protocol, please refer to Tang et al. (2021).

【 授权许可】

Unknown