Assessment of dopaminergic neuron degeneration in a C. elegans model of Parkinson’s disease

STAR Protocols

Tanima SenGupta¹  Nektarios Tavernarakis²  Hilde Nilsen³  Konstantinos Palikaras⁴ 

[1] Corresponding author;Department of Clinical Molecular Biology, Akershus University Hospital, Lørenskog, Norway;Department of Clinical Molecular Biology, University of Oslo, Oslo, Norway;Department of Physiology, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece;

关键词: Cell Biology;    Model Organisms;    Molecular Biology;    Neuroscience;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Transgenic Caenorhabditis elegans that expresses the full-length wild-type human α-synuclein in dopaminergic neurons provides a well-established Parkinson’s disease (PD) nematode model. Here, we present a detailed protocol to monitor and dissect the molecular underpinnings of age-associated neurodegeneration using this PD nematode model. This protocol includes preparation of nematode growth media and bacterial food sources, as well as procedures for nematode growth, synchronization, and treatment. We then describe procedures to assess dopaminergic neuronal death in vivo using fluorescence imaging.For complete details on the use and execution of this protocol, please refer to SenGupta et al. (2021).

【 授权许可】

Unknown