Ιn vivo imaging of DNA-bound minichromosome maintenance complex in embryonic mouse cortex

STAR Protocols

Stavros Taraviras¹  Argyro Kalogeropoulou²  Zoi Lygerou³  Maria Mougkogianni³  Nickolaos Nikiforos Giakoumakis³ 

[1] Advanced Digital Microscopy Facility, Institute for Research in Biomedicine-IRB Barcelona, 08028 Barcelona, Spain;Corresponding author;Department of Physiology, School of Medicine, University of Patras, Rio, Patras 26504, Greece;

关键词: Cell Biology;    Microscopy;    Model Organisms;    Molecular Biology;    Neuroscience;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The recruitment of the minichromosome maintenance complex (MCM) on DNA replication origins is a critical process for faithful genome duplication termed licensing. Aberrant licensing has been associated with cancer and, recently, with neurodevelopmental diseases. Investigating MCM loading in complicated tissues, such as brain, remains challenging. Here, we describe an optimized approach for the qualitative and quantitative analysis of DNA-bound MCMs in the developing mouse cortex through direct imaging, offering an innovative insight into the research of origin licensing in vivo.

【 授权许可】

Unknown