Protocol to assess the impact of early-life antibiotic exposure on murine longevity

STAR Protocols

Feargal J. Ryan¹  Saoirse C. Benson²  Yee C. Tee³  David J. Lynn³  Miriam A. Lynn³ 

[1] Corresponding author;Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, Australia;Precision Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA 5001, Australia;

关键词: Sequencing;    Immunology;    Metabolism;    Microbiology;    Model Organisms;    Molecular Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure.For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).

【 授权许可】

Unknown