Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens

South African Journal of Chemical Engineering

Ana Afonso¹  Fernando M. Nunes²  Guilhermina Marques³  Juliana Garcia⁴  Conceição Fernandes⁴  Maria José Saavedra⁵ 

[1] Authors to whom correspondence should be addressed.;CIMO – Centro de Investigação de Montanha, Instituto Politécnico de Bragança 5300-253 Bragança, Portugal;CIMO – Centro de Investigação de Montanha, Instituto Politécnico de Bragança 5300-253 Bragança, Portugal;CITAB – Centro de Investigação e Tecnologias Agroambientais e Biológicas, Universidade de Trás-os-Montes e Alto Douro, 5001- 801 Vila Real, Portugal;CQ – Centro de Química, Universidade de Trás- os-Montes e Alto Douro, 5001-801 Vila Real, Portugal;

关键词: Antibiotic resistance;    Donko;    Koshin;    Klebsiella pneumoniae;    Shiitake;    Staphylococcus aureus;   

DOI  :  

来源: DOAJ

【 摘 要 】

The problematic increase in multidrug-resistant bacteria translates into the urgent need to discover novel and effective antimicrobial substances. Herein, mushrooms could be a promising alternative of natural source of new antimicrobials. The present work aimed to compare the phytochemical composition and antimicrobial activity of methanol and aqueous crude extracts of Lentinula edodes var. Koshin and Donko. Disk diffusion method was used to screen the antimicrobial activity and to assess the synergistic effect of the mushroom extracts. Microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Phytochemical characterization of mushrooms extracts was achieved by analysis of total phenols, ortho-diphenols content and its antioxidant activity. The results noticed a positive relation between phenolic compounds content, antioxidant activity, and antimicrobial capacity of the mushroom's extracts. The L. edodes var. Koshin aqueous extracts, which contained the highest amount of total phenolic compounds, exhibited the highest scavenging capacity of ABTS which, in turns, exhibited the highest antimicrobial efficacy in inhibiting the growth of methicillin-resistant Staphylococcus aureus. Moreover, the combination between mushrooms extracts and commercial antibiotics showed favorable synergistic effects against tested bacteria. These results suggest that L. edodes var. Koshin may represent an important and valuable therapeutic source of compounds to be used against multidrug-resistant bacteria.

【 授权许可】

Unknown