Enhanced Cell Growth of Adipocyte-Derived Mesenchymal Stem Cells Using Chemically-Defined Serum-Free Media

International Journal of Molecular Sciences

Byoung Jun Park¹  Young-Deug Kim¹  Christine Youn²  Young Kee Shin²  Sungyoul Hong³  Sang Gyu Park⁴  Jeong Hyun Kim⁴  Myung-Suk Lee⁴  Jongchan Ahn⁴ 

[1] ABION Inc., Hanhwa Biz Metro Building, 242 Digital-ro, Guro-gu, Seoul 152-733, Korea;College of Pharmacy, Seoul National University, Kwanak-ro, Kwanak-gu, Seoul 151-742, Korea;Research Institute of Pharmaceutical Sciences, Seoul National University, Kwanak-ro, Kwanak-gu, Seoul 151-742, Korea;Xcell Therapeutics, Hanhwa Biz Metro Building, 242 Digital-ro, Guro-gu, Seoul 152-733, Korea;

关键词: mesenchymal stem cell;    multipotency;    chemically-defined medium;    proliferation;    differentiation;    secretome;   

DOI  :  10.3390/ijms18081779

来源: DOAJ

【 摘 要 】

The multipotency and anti-inflammatory effects of mesenchymal stem cells (MSCs) make them attractive for cell therapy in regenerative medicine. A large number of MSCs is required for efficient therapy owing to the low homing efficiency of MSCs to target sites. Furthermore, owing to limitations in obtaining sufficient amounts of MSCs, in vitro expansion of MSCs that preserves their differentiation and proliferative potential is essential. The animal factor included in culture media also limits clinical application. In this study, adipose-derived MSCs showed a significantly higher proliferation rate in STK2, a chemically-defined medium, than in DMEM/FBS. The expression of MSC surface markers was increased in the culture using STK2 compared to that using DMEM/FBS. Tri-lineage differentiation analyses showed that MSCs cultured in STK2 were superior to those cultured in DMEM/FBS. In addition, MSCs cultured in STK2 showed a reduced senescence rate, small and homogenous cell size, and were more genetically stable compared to those cultured in DMEM/FBS. Furthermore, secretome analysis showed that the expression of factors related to proliferation/migration, anti-inflammation, and differentiation were increased in STK2 culture medium compared to DMEM/FBS. Taken together, these results suggest that culture using STK2 medium offers many advantages through which it is possible to obtain safer, superior, and larger numbers of MSCs.

【 授权许可】

Unknown