Quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis

Genome Biology

Stanislav Dusko Ehrlich¹  Emmanuelle Le Chatelier¹  Chengping Wen²  Haichang Li²  Zhixing He²  Zhijun Xie²  Jia Zhou²  Yongsheng Fan²  Dawei Wang²  Changfeng Hu²  Tiejuan Shao²  Wendi Zhong³  Shunfeng Cai³  Linshuang Zhang³  Chunyan Wu³  Yun Huang³  Qian Xu³  Nan Qin³  Zhijun Zheng³  Maxime Breban⁴  Lin Liu⁵ 

[1] INRA, Institut National de la Recherche Agronomique, Metagenopolis;Institute of Basic Research in Clinical Medicine, College of Basic Medical Science, Zhejiang Chinese Medical University;Realbio Genomics Institute;Rheumatology Division, Ambroise-Paré Hospital, AP-HP;State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Infectious Diseases, the First Affiliated College of Medicine, Zhejiang University;

关键词: Ankylosing spondylitis;    Human gut microbiome;    Biomarkers;    Pathogenesis;   

DOI  :  10.1186/s13059-017-1271-6

来源: DOAJ

【 摘 要 】

Abstract Background The assessment and characterization of the gut microbiome has become a focus of research in the area of human autoimmune diseases. Ankylosing spondylitis is an inflammatory autoimmune disease and evidence showed that ankylosing spondylitis may be a microbiome-driven disease. Results To investigate the relationship between the gut microbiome and ankylosing spondylitis, a quantitative metagenomics study based on deep shotgun sequencing was performed, using gut microbial DNA from 211 Chinese individuals. A total of 23,709 genes and 12 metagenomic species were shown to be differentially abundant between ankylosing spondylitis patients and healthy controls. Patients were characterized by a form of gut microbial dysbiosis that is more prominent than previously reported cases with inflammatory bowel disease. Specifically, the ankylosing spondylitis patients demonstrated increases in the abundance of Prevotella melaninogenica, Prevotella copri, and Prevotella sp. C561 and decreases in Bacteroides spp. It is noteworthy that the Bifidobacterium genus, which is commonly used in probiotics, accumulated in the ankylosing spondylitis patients. Diagnostic algorithms were established using a subset of these gut microbial biomarkers. Conclusions Alterations of the gut microbiome are associated with development of ankylosing spondylitis. Our data suggest biomarkers identified in this study might participate in the pathogenesis or development process of ankylosing spondylitis, providing new leads for the development of new diagnostic tools and potential treatments.

【 授权许可】

Unknown