Characterization of Ambrette Seed Oil and Its Mode of Action in Bacteria

Molecules

Savariar Vincent¹  Vannam Dinesh-Kumar¹  Young Kyoon Oh²  Seong Ho Choi³  Rajaraman Bharanidharan⁴  Badathala Vijayakumar⁵  Selvaraj Arokiyaraj⁶  Yoonseok Lee⁶  Kyoung Hoon Kim⁶  Villianur Ibrahim Hairul-Islam⁷ 

[1] Centre for Environment Research and Development, Loyola College, Chennai 600034, India;Department of Animal Nutrition and Physiology, National Institute of Animal Science,Rural Development Administration, Suwon 441706, Korea;Department of Animal Science, Chungbuk National University, Chunbuk 361763, Korea;Department of Biotechnology, Vel Tech High Tech Dr.Rangarajan Dr.Sakunthala Engineering College (Anna University), Chennai 600062, India;Department of Chemistry, Vel Tech High Tech Dr.Rangarajan Dr.Sakunthala Engineering College (Anna University), Chennai 600062, India;Institute of Green Bio Science & Technology, Seoul National University,Pyeongchang-daero 232916, Korea;Pondicherry Centre for Biological Sciences, Pondicherry 605005, India;

关键词: ambrette seed oil;    antibacterial activity;    chemical composition;    docking;    DHFR;   

DOI  :  10.3390/molecules20010384

来源: DOAJ

【 摘 要 】

In the present study, chemical composition and the antibacterial mechanism of ambrette seed oil are investigated. Chemical composition of the oil was analysed by gas chromatography-mass spectrometry (GC-MS). Thirty-five compounds were identified and the major compounds were found to be farnesol acetate (51.45%) and ambrettolide (12.96%). The antibacterial activity was performed by well diffusion assay and the mechanisms were studied by measuring the alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and protein leakage assays. The antibacterial effect of the ambrette seed oil showed inhibitory effect against Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis. The LDH activity was high in all tested bacteria compared with control, whereas the ALP and protein concentrations were also increased in E. faecalis. Molecular docking revealed the ligands farnesol acetate and ambrettolide had satisfactory binding energy towards the beta lactamase TEM-72 and dihydrofolate reductase (DHFR) protein. Due to its better antibacterial properties, the ambrette seed oil could be used as a source of antibacterial agents.

【 授权许可】

Unknown