A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes

Beilstein Journal of Nanotechnology

Matthias F. Schneider¹  Dominik Breyer²  Florian G. Strobl²  Phillip Link²  Achim Wixforth²  Christoph Bräuchle³  Adriano A. Torrano³ 

[1] Department for Mechanical Engineering, Boston University, Boston, MA 02215, USA;Lehrstuhl für Experimentalphysik I, Universität Augsburg, 86159 Augsburg, Germany;Nanosystems Initiative Munich NIM, Schellingstr. 4, 80799 Munich, Germany;

关键词: acoustic streaming;    cellular uptake;    flow;    nanoparticles;    sedimentation;    shear;    surface acoustic wave (SAW);   

DOI  :  10.3762/bjnano.6.41

来源: DOAJ

【 摘 要 】

Static conditions represent an important shortcoming of many in vitro experiments on the cellular uptake of nanoparticles. Here, we present a versatile microfluidic device based on acoustic streaming induced by surface acoustic waves (SAWs). The device offers a convenient method for introducing fluid motion in standard cell culture chambers and for mimicking capillary blood flow. We show that shear rates over the whole physiological range in sample volumes as small as 200 μL can be achieved. A precise characterization method for the induced flow profile is presented and the influence of flow on the uptake of Pt-decorated CeO2 particles by endothelial cells (HMEC-1) is demonstrated. Under physiological flow conditions the particle uptake rates for this system are significantly lower than at low shear conditions. This underlines the vital importance of the fluidic environment for cellular uptake mechanisms.

【 授权许可】

Unknown