A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies

STAR Protocols

Cynthia Faubert¹  Garrie Peng²  Siegfried Hapfelmeier²  Irah L. King³  Gabriel A. Russell⁴  Elena F. Verdu⁴ 

[1] Corresponding author;Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada;McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, QC H4A 3J1, Canada;Meakins-Christie Laboratories, Department of Medicine, McGill University Health Centre, Montreal, QC H4A 3J1, Canada;

关键词: Cell culture;    Health Sciences;    Immunology;    Microbiology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.

【 授权许可】

Unknown