A touch transfer assay to determine surface transmission of highly pathogenic viruses

STAR Protocols

Eike Steinmann¹  Yannick Brüggemann¹  Daniel Todt¹  Toni Luise Meister¹  Barbora Tamele²  John Howes² 

[1] Department for Molecular and Medical Virology, 44801 Bochum, Germany;European Central Bank (ECB), 60314 Frankfurt am Main, Germany;

关键词: Biotechnology and bioengineering;    Cell Biology;    Cell culture;    Health Sciences;    Immunology;    Material sciences;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Transmission via fomites poses a major dissemination route for many human pathogens, particularly because of transfer via fingertips. Here, we present a protocol to investigate direct transfer of infectious agents from fomites to humans via naked fingertips. The protocol is suitable for pathogens requiring highest biosafety levels (e.g., SARS-CoV-2). We used an artificial skin to touch a defined volume of virus suspension and subsequent quantification of infectious entities allows quantitative measurement of transfer efficiency and risk assessment.For complete information on the generation and use of this manuscript, please refer to Todt et al. (2021).

【 授权许可】

Unknown