Protocol for engineering and validating a synthetic mitochondrial intermembrane bridge in mammalian cells

STAR Protocols

卷:3

Martonio Ponte Viana¹  Oleh Khalimonchuk²  Gunjan Purohit² 

[1] Corresponding author;

[2] Department of Biochemistry, University of Nebraska, Lincoln, NE 68503, USA;

关键词: Biotechnology and bioengineering;    Cell Biology;    Cell culture;    Cell isolation;    Cell Membrane;    Flow Cytometry/Mass Cytometry;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Membrane contact sites are recognized as critical means of intercompartmental communication. Here, we describe a protocol for engineering and validating a synthetic bridge between the inner and outer mitochondrial membranes to support functioning of the endogenous mitochondrial contact site and cristae organizing system (MICOS). A chimeric protein, MitoT, is stably expressed in cultured mammalian cells to bridge the mitochondrial membranes. This approach can be a valuable tool to study the function of the MICOS complex and associated proteins.For complete details on the use and execution of this protocol, please refer to Viana et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

【 授权许可】

Unknown