Functional assays with human patient-derived enteroid monolayers to assess the human gut barrier

STAR Protocols

卷:2

Ayden G. Fonseca¹  Soumita Das¹  Courtney Tindle²  Pradipta Ghosh²  Ibrahim M. Sayed³ 

[1] HUMANOID CoRE, University of California, San Diego, CA 92093, USA;

[2] Department of Cellular and Molecular Medicine, University of California, San Diego, CA 92093, USA;

[3] Department of Pathology, University of California, San Diego, CA 92093, USA;

关键词: Cell Biology;    Cell culture;    High Throughput Screening;    Immunology;    Microbiology;    Stem Cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Here, we describe the use of polarized patient enteroid-derived monolayers (EDMs) to assess the impact of e-cigarettes on the human gut barrier. These EDMs can be adapted to culture in a 96-well plate for high-throughput screening. We model the effect of e-cigarettes by combining pathogens, enteroids, and e-cigarette vapor-infused media and assess gut barrier integrity, bacterial internalization, and inflammatory response of the gut epithelium. This protocol can be used to assess the effects of e-cigarette components on gut functions.For complete details on the use and execution of this protocol, please refer to Sharma et al. (2021).

【 授权许可】

Unknown