Study of potential xanthine oxidase inhibitors: In silico and in vitro biological activity

Bangladesh Journal of Pharmacology

Kuppusamy Asokkumar¹  Thirumalaisamy Sivashanmugam¹  Puliyath Jagannath¹  Muthuswamy Umamaheswari¹  Varadharajan Subhadradevi¹  Arumugam Madeswaran¹ 

[1] Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore 641044, Tamil Nadu;

关键词: Allopurinol;    AutoDock 4.2;    Docking;    Enzyme kinetics;    Xanthine oxidase;   

DOI  :  10.3329/bjp.v6i2.9175

来源: DOAJ

【 摘 要 】

In an attempt to develop potent anti gout agents, coumarin derivatives and polyphenolic compounds were selected for present study. The docking energy of 2-benzyl coumarin was found to be -7.50 kcal/mol which was less than that of the standard allopurinol (-4.47 kcal/mol). All the selected compounds were found to exhibit lower binding energy (-7.50 to -4.68 kcal/mol) than allopurinol. Docking results confirm that selected compounds showed greater inhibition of xanthine oxidase due to their active binding sites. In xanthine oxidase assay, IC50 value of 2-benzyl coumarin was found to be 26 ± 1.16 µg/mL, whereas that of allopurinol was 24 ± 0.28 µg/mL. All the compounds exhibited IC50 values ranging between 26 ± 1.16 to 58 ± 0.74 µg/mL.  In enzyme kinetic studies, coumarin derivatives showed competitive and polyphenolic compounds showed non competitive type of enzyme inhibition. It can be concluded that coumarin derivatives could be a remedy for the treatment of gout and related inflammatory disorders.

【 授权许可】

Unknown