Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

Acta Cirurgica Brasileira

André Roberto Bussmann¹  Marcos Antônio Marton Filho¹  Marília Pinheiro Módolo¹  Renata Pinheiro Módolo¹  Patrícia Amado¹  Maria Aparecida Custódio Domingues¹  Yara Marcondes Machado Castiglia¹  Norma Sueli Pinheiro Módolo¹ 

关键词: Ischemia;    Acute Kidney Injury;    Allopurinol;    Interleukin-18;    Biomarkers;    Pharmacological;    Rats;   

DOI  :  10.1590/S0102-86502014000800006

来源: SciELO

【 摘 要 】

PURPOSE:To investigate whether allopurinol exerts a protective effect on kidneys by measuring new kidney injury biomarkers (NGALp, NGALu, KIM 1 and IL 18) and analysing the renal function and histology in uninephrectomised rats subjected to ischaemia-reperfusion injury.METHODS: Thirty two Wistar rats were randomly allocated to four groups: Sham (S): laparotomy; Control (C): laparotomy and ischaemia-reperfusion in the left kidney; Control Allopurinol (CA): laparotomy and allopurinol at a dose of 100mg·kg1·d 1; and Allopurinol (A): laparotomy ischaemia-reperfusion in the left kidney and allopurinol at a dose of 100mg·kg 1·d1. The NGALp, NGALu, KIM 1, IL 18 and creatinine levels and the kidney histology were analysed. The significance level was established as p<0.05.RESULTS:Creatinine level increased in all the groups, with A ≈ C > S ≈ CA. The NGALp, NGALu and IL 18 levels exhibited similar behaviour in all the groups. KIM 1 was higher in group A than C and showed intermediate values in groups S and CA. Severity of injury in the left kidney was greater in groups C and A compared to S and CA.CONCLUSION:Allopurinol did not exert protective or damaging effects on the kidneys of rats subjected to ischaemia-reperfusion injury.

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