Development of a recombinant protein-based ELISA for detection of antibodies against bovine foamy virus

Journal of Veterinary Research

Rudzki Marcin¹  Osiński Zbigniew²  Kuźmak Jacek³  Materniak-Kornas Magdalena³ 

[1] Chair and Department of Jaw Orthopaedics, Medical University, 20-081 Lublin, Poland;Departament of Hygiene of Feeding Stuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland;Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland;

关键词: cattle;    bovine foamy virus;    seroprevalence;    bayesian estimation;   

DOI  :  10.1515/jvetres-2017-0034

来源: DOAJ

【 摘 要 】

Introduction: Infections with bovine foamy virus (BFV) were found in many countries but there is a lack of large-scale surveys on the prevalence of BFV among dairy cattle. The aim of this study was to develop and validate the recombinant Gag protein-based ELISA and to estimate the prevalence of antibodies against BFV. Material and Methods: Gag coding region from BFV was cloned into expression vector pT7Arg-STOP, which expressed a high level of recombinant Gag protein from E.coli. The ELISA was standardised, and the cut-off value and sensitivity and specificity of the test were calculated using a receiver operating characteristic and Bayesian estimation. Results: A total of 3,051 serum samples were tested by ELISA and 939 (30.8%) sera were recognised as positive. When Bayesian approach was used, the overall true BFV prevalence was 29.7% (95% CI: 25.9-33.4%). Conclusion: Expressed Gag protein of BFV has been used successfully as an antigen for ELISA. Eventually, this study provides basic information about the epidemiological status of infection with BFV in dairy cattle in Poland, which can be used for further studies on dissemination and transmission of BFV infection.

【 授权许可】

Unknown