Epidemiology and genetic characterization of BVDV, BHV-1, BHV-4, BHV-5 andBrucella spp. infections in cattle in Turkey

Journal of Veterinary Medical Science

Serkal GAZYAGCI¹  Ahmet Kursat AZKUR²  Muhammet Eren ASLAN² 

[1] Department of Internal Medicine, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale 71450, Turkey;Department of Virology, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale 71450, Turkey

关键词: abortion agent;    cattle;    phylogenetic analysis;    seroprevalence;   

DOI  :  10.1292/jvms.14-0657

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(58)The aim of the study was to determine the epidemiological data of bovine viral diarrheavirus (BVDV), bovine herpesvirus-1 (BHV-1), bovine herpesvirus-4 (BHV-4), bovineherpesvirus-5 (BHV-5) and Brucella–associated cattle that were previouslyreported to have abortion and infertility problems in Ankara, Corum, Kirikkale and Yozgatprovinces, Turkey. Whole blood and sera samples were obtained from 656 cattle, andantibodies against Brucella spp. were detected in 45 (6.86%) and 41(6.25%) animals by Rose Bengal plate and serum tube agglutination tests, respectively. Theseropositivity rates against BVDV, BHV-1 and BHV-4 were 70.89%, 41.3% and 28.78%,respectively. RT-PCR and PCR were performed to detect RNA and DNA viruses in bloodsamples, respectively. The BVDV 5�?-untranslated region and BHV-1 gB gene detected in thisstudy were phylogenetically analyzed. The BVDV strains analyzed in this study were closelyrelated to those previously reported from Turkey. The nucleotide sequence from the BHV-1strain detected in this study is the first nucleotide sequence of BHV-1 circulating inthis area of Turkey deposited in the GenBank. The presence of Brucellaspp. and prevalence of BHV-1, BHV-4 and BVDV in cattle should be further investigatedthroughout these regions.

【 授权许可】

Unknown