Isolation of bovine foamy virus in Japan

The Journal of Veterinary Medical Science

Yuma HACHIYA¹  Kumiko KIMURA² 

[1] Department of Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-0880, Japan;National Institute of Animal Health, National Agriculture and Food Research Organization (NARO) 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan

关键词: bovine foamy virus;    cattle;    retrovirus;    spumavirus;    syncytium;   

DOI  :  10.1292/jvms.18-0121

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Bovine foamy virus (BFV) is endemic in many countries, but has not been reported in Japan. A syncytium-forming virus was isolated from peripheral blood leukocytes of clinically healthy cattle on a farm in Kanagawa prefecture during a periodic epidemiological survey of viral diseases. The isolate was propagated in primary fetal bovine muscle cells and subsequently passaged in Madin–Darby bovine kidney cells. Since the isolate appeared to be distinct from the viruses with syncytium-forming ability previously isolated in Japan, we attempted to identify it using genomic analyses and electron microscopy. A phylogenetic analysis revealed that the isolate belongs to the bovine foamy virus cluster and is highly similar to a BFV strain isolated in China. A sero-epidemiological survey was performed using agar gel immunodiffusion test with the isolated virus as the antigen, and five of the 57 cattle tested were found to be seropositive.

【 授权许可】

Unknown