Exploring the whole standard operating procedure for phage therapy in clinical practice

Journal of Translational Medicine

Tingting Feng¹  Xiaokui Guo²  Li Li³  Zelin Cui³ 

[1] Department of Clinical Pharmacy, Shanghai General Hospital;Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine;Department of Laboratory Medicine, Shanghai General Hospital;

关键词: Phage therapy;    Clinical practice;    Streamline;    Standard operating procedures;    Antibiotic resistance;   

DOI  :  10.1186/s12967-019-2120-z

来源: DOAJ

【 摘 要 】

Abstract We have entered the post-antibiotic era. Phage therapy has recently been given renewed attention because bacteriophages are easily available and can kill bacteria. Many reports have demonstrated successful phage treatment of bacterial infection, whereas some studies have shown that phage therapy is not as effective as expected. In general, establishment of a standard operating procedure will ensure the success of phage therapy. In this paper, the whole operating procedure for phage therapy in clinical practice is explored and analyzed to comprehensively understand the success of using phage for the treatment of bacterial infectious disease in the future. The procedure includes the following: enrollment of patients for phage therapy; establishment of phage libraries; pathogenic bacterial isolation and identification; screening for effective phages against pathogenic bacteria; phage formulation preparation; phage preparation administration strategy and route; monitoring the efficacy of phage therapy; and detection of the emergence of phage-resistant strains. Finally, we outline the whole standard operating procedure for phage therapy in clinical practice. It is believed that phage therapy will be used successfully, especially in personalized medicine for the treatment of bacterial infectious diseases. Hopefully, this procedure will provide support for the entry of phage therapy into the clinic as soon as possible.

【 授权许可】

Unknown