Isolation of antimicrobial producing Actinobacteria from soil samples

Saudi Journal of Biological Sciences

Saleh A. Kabli¹  Ihab Mohamed Moussa²  Ayman Salem Mubarak²  Ashgan Mohamed Hessain³  Hassan A. Hemeg⁴  Jakeen Kamal El Jakee⁵  Ahmed Adel Seida⁵  Mahmoud Darderi El-Hariri⁵  Afaf Ahmed Elbendary⁵ 

[1] Department of Biology, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia;Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;Department of Health Science, College of Applied Studies and Community Service, King Saud University, P.O. Box 22459, Riyadh 11495, Saudi Arabia;Department of Medical Technology/Microbiology, College of Applied Medical Science, Taibah University, Madinah, Saudi Arabia;Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Cairo University, Giza 11221, Egypt;

关键词: Actinobacteria;    Antibacteria;    Antifungal;    Kocuria;    Soil;    Streptomyces;   

DOI  :  10.1016/j.sjbs.2017.05.003

来源: DOAJ

【 摘 要 】

Emergence of multidrug resistant bacteria has made the search for novel bioactive compounds from natural and unexplored habitats a necessity. Actinobacteria have important bioactive substances. The present study investigated antimicrobial activity of Actinobacteria isolated from soil samples of Egypt. One hundred samples were collected from agricultural farming soil of different governorates. Twelve isolates have produced activity against the tested microorganisms (S. aureus, Bacillus cereus, E. coli, K. pneumoniae, P. aeruginosa, S. Typhi, C. albicans, A. niger and A. flavus). By VITEK 2 system version: 07.01 the 12 isolates were identified as Kocuria kristinae, Kocuria rosea, Streptomyces griseus, Streptomyces flaveolus and Actinobacteria. Using ethyl acetate extraction method the isolates culture’s supernatants were tested by diffusion method against indicator microorganisms. These results indicate that Actinobacteria isolated from Egypt farms could be sources of antimicrobial bioactive substances.

【 授权许可】

Unknown