Biocontrol potential of Streptomyces sp. CACIS-1.5CA against phytopathogenic fungi causing postharvest fruit diseases

Egyptian Journal of Biological Pest Control

Zahaed Evangelista-Martínez¹  Élida Gastélum-Martínez¹  Erika Anahí Contreras-Leal¹  Luis Fernando Corona-Pedraza¹ 

[1] Center for Research and Assistance in Technology and Design of the State of Jalisco AC (CIATEJ), Southeast Unit, Tablaje Catastral 31264 km 5.5 Carretera Sierra Papacal-Chuburná Puerto, Parque Científico y Tecnológico de Yucatán, CP 97302, Mérida, Yucatán, Mexico;

关键词: Actinobacteria;    Streptomyces;    Biological control;    Fungal phytopathogens;    Antifungal;    Antagonistic activity;   

DOI  :  10.1186/s41938-020-00319-9

来源: Springer

【 摘 要 】

BackgroundFungi are one of the microorganisms that cause most damage to fruits worldwide, affecting their quality and consumption. Chemical controls with pesticides are used to diminish postharvest losses of fruits. However, biological control with microorganisms or natural compounds is an increasing alternative to protect fruits and vegetables. In this study, the antifungal effect of Streptomyces sp. CACIS-1.5CA on phytopathogenic fungi that cause postharvest tropical fruit rot was investigated.Main bodyAntagonistic activity was evaluated in vitro by the dual confrontation over fungal isolates obtained from grape, mango, tomato, habanero pepper, papaya, sweet orange, and banana. The results showed that antagonistic activity of the isolate CACIS-1.5CA was similar to the commercial strain Streptomyces lydicus WYEC 108 against the pathogenic fungi Colletotrichum sp., Alternaria sp., Aspergillus sp., Botrytis sp., Rhizoctonia sp., and Rhizopus sp. with percentages ranging from 30 to 63%. The bioactive extract obtained from CACIS-1.5 showed a strong inhibition of fungal spore germination, with percentages ranging from 92 to 100%. Morphological effects as irregular membrane border, deformation, shrinkage, and collapsed conidia were observed on the conidia. Molecularly, the biosynthetic clusters of genes for the polyketide synthase (PKS) type I, PKS type II, and NRPS were detected in the genome of Streptomyces sp. CACIS-1.5CA.ConclusionsThis study presented a novel Streptomyces strain as a natural alternative to the use of synthetic fungicides or other commercial products having antagonistic microorganisms that were used in the postharvest control of phytopathogenic fungi affecting fruits.

【 授权许可】

CC BY