CircMCTP2 (has-circ-0000658) facilitates the proliferation and metastasis of bladder carcinoma through modulating the miR-498/murine double minute-2 axis

Bioengineered

Wenjie Hou¹  Lijuan Shi²  Zonghao Zhu²  Qiao Gu²  Huan Liu³  Xiaozhou He⁴ 

[1] Department of Gynecology and Obstetrics, Dushu Lake Hospital Affiliated to Soochow University (Medical Center of Soochow University), Suzhou, P.R. China;Department of Gynecology and Obstetrics, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China;Department of Pathology, Changzhou Hospital of Traditional Chinese Medicine, Changzhou, P.R. China;Department of Urology, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China;

关键词: Bladder carcinoma;    circMCTP2;    miR-498;    MDM2;   

DOI  :  10.1080/21655979.2022.2054161

来源: DOAJ

【 摘 要 】

CircMCTP2 is a novel circRNA, which is associated with various kinds of malignant tumors progression, such as gastric cancer. However, the function of circMCTP2 in bladder carcinoma (BC) has no idea. The purpose of this study was tantamount to functionally dissect circMCTP2 in the progression of BC. In our study, circMCTP2 expression was strongly increased in BC tissues and cell lines. High expression of circMCTP2 predicted a poor prognosis of BC patients. CircMCTP2 deficiency impaired the cell growth, migration as well as invasive ability of BC cell lines (J82 and T24). In vivo, circMCTP2 deficiency cut the tumor growth rates and the tumor weight. In BC cells, circMCTP2 deficiency enhanced the translation of E-cadherin, while diminishing the translation of N-cadherin, Vimentin, and Snail. Moreover, circMCTP2 acted as a sponge of miR-498 to regulate murine double minute-2 (MDM2) expression. In BC tissues, a negative correlation was observed between the expression levels of circMCTP2 and miR-498. Additionally, either miR-498 silencing or MDM2 over-expression augmented the carcinogenic action of circMCTP2 on BC. In conclusion, our study showed that circMCTP2 regulates the expression of MDM2 by sponging miR-498 to promote the development of BC. These findings offer a new strategy for early diagnosis of BC and its therapeutics.

【 授权许可】

Unknown