Long non-coding RNA UFC1 promotes gastric cancer progression by regulating miR-498/Lin28b

Journal of Experimental & Clinical Cancer Research

Pengcheng Jiang¹  Wenrong Xu²  Min Fu²  Lei Pan²  Yu Zhang²  Xu Zhang²  Zhenhua Huang²  Hui Qian²  Hui Shi²  Wei Liang²  Xueyan Zang²  Jianmei Gu³  Jibin Liu³ 

[1] Institute of Digestive Diseases of Jiangsu University, The Affiliated People’s Hospital of Jiangsu University;Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University;Tumor Institute, Nantong Tumor Hospital;

关键词: UFC1;    Lin28b;    miR-498;    Gastric cancer;    Progression;    Biomarker;   

DOI  :  10.1186/s13046-018-0803-6

来源: DOAJ

【 摘 要 】

Abstract Background Long non-coding RNAs (lncRNAs) have emerged as important regulators of human cancers. However, the functional roles of lncRNAs and the mechanisms responsible for their aberrant expression in gastric cancer (GC) have not been well characterized. Methods In this study, we examined the expression of lncRNA UFC1 in GC by qRT-PCR and explored its correlation with clinicopathological parameters. In vitro cell functional assays and in vivo animal studies were performed to determine the roles of UFC1 in GC progression. Results UFC1 was elevated and predicted poorer prognosis in GC. UFC1 knockdown inhibited while UFC1 overexpression promoted GC cell proliferation, migration, and invasion. UFC1 bound to miR-498 to antagonize its tumor suppressive effect on Lin28b. Suppression of Lin28b by miR-498 could be rescued by UFC1 overexpression, whereas Lin28b overexpression partially rescued UFC1 knockdown-mediated inhibition of GC cell function. Lin28b expression was increased in GC and suggested a co-expression pattern with UFC1. Conclusions UFC1 has a promoting role in GC progression, at least in part, by acting as a miR-498 sponge and derepressing Lin28b expression, which would provide a novel biomarker for GC diagnosis and prognosis and offer a potential target for GC therapy.

【 授权许可】

Unknown