Exercise Rescues Gene Pathways Involved in Vascular Expansion and Promotes Functional Angiogenesis in Subcutaneous White Adipose Tissue

International Journal of Molecular Sciences

JohnF. Keaney¹  Heather Learnard¹  Shashi Kant¹  SiobhanM. Craige²  Raziel Rojas-Rodriguez³  Anand Desai³  Silvia Corvera³  SoYun Min³  Tiffany DeSouza³  Olga Gealikman³ 

[1] Division of Cardiovascular Medicine, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA;Human Nutrition, Food, and Exercise Department, Virginia Tech, Blacksburg, VA 24060, USA;Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA;

关键词: adipose tissue;    angiogenesis;    exercise;    glucose homeostasis;    high-fat diet;    metabolism;   

DOI  :  10.3390/ijms20082046

来源: DOAJ

【 摘 要 】

Exercise mitigates chronic diseases such as diabetes, cardiovascular diseases, and obesity; however, the molecular mechanisms governing protection from these diseases are not completely understood. Here we demonstrate that exercise rescues metabolically compromised high fat diet (HFD) fed mice, and reprograms subcutaneous white adipose tissue (scWAT). Using transcriptomic profiling, scWAT was analyzed for HFD gene expression changes that were rescued by exercise. Gene networks involved in vascularization were identified as prominent targets of exercise, which led us to investigate the vasculature architecture and endothelial phenotype. Vascular density in scWAT was found to be compromised in HFD, and exercise rescued this defect. Similarly, angiogenic capacity as measured by ex vivo capillary sprouting was significantly promoted with exercise. Together, these data demonstrate that exercise enhances scWAT vascularization and functional capacity for angiogenesis, and can prevent the detrimental effects of HFD. The improvement in these indices correlates with improvement of whole-body metabolism, suggesting that scWAT vascularization may be a potential therapeutic target for metabolic disease.

【 授权许可】

Unknown