RNA-Seq Time Series of Vitis vinifera Bud Development Reveals Correlation of Expression Patterns with the Local Temperature Profile

Plants

Prisca Viehöver¹  Bernd Weisshaar¹  Boas Pucker¹  Daniela Holtgräwe¹  Sarah Becker¹  Ludger Hausmann²  Reinhard Töpfer²  Anna Schwandner² 

[1] Genetics and Genomics of Plants, Faculty of Biology and Center for Biotechnology (CeBiTec), Bielefeld University, 33615 Bielefeld, Germany;Julius Kühn-Institut (JKI), Institute for Grapevine Breeding Geilweilerhof, 76833 Siebeldingen, Germany;

关键词: grapevine;    ontogenesis;    gene expression;    AP1;    DRM1;    MYB;   

DOI  :  10.3390/plants9111548

来源: DOAJ

【 摘 要 】

Plants display sophisticated mechanisms to tolerate challenging environmental conditions and need to manage their ontogenesis in parallel. Here, we set out to generate an RNA-Seq time series dataset throughout grapevine (Vitis vinifera) early bud development. The expression of the developmental regulator VviAP1 served as an indicator of the progression of development. We investigated the impact of changing temperatures on gene expression levels during the time series and detected a correlation between increased temperatures and a high expression level of genes encoding heat-shock proteins. The dataset also allowed the exemplary investigation of expression patterns of genes from three transcription factor (TF) gene families, namely MADS-box, WRKY, and R2R3-MYB genes. Inspection of the expression profiles from all three TF gene families indicated that a switch in the developmental program takes place in July which coincides with increased expression of the bud dormancy marker gene VviDRM1.

【 授权许可】

Unknown