Sap Flow Disruption in Grapevine Is the Early Signal Predicting the Structural, Functional, and Genetic Responses to Esca Disease

Frontiers in Plant Science

Jean-Christophe Domec¹  Patrice Rey²  Emilie Bruez²  Lucia Guérin-Dubrana²  Sylvie Bastien²  Loris Ouadi³  Amira Yacoub³  Cindy Coppin⁵  Florence Fontaine⁵ 

[1] Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine, Bordeaux Sciences Agro, INRAE UMR1391 Interactions Sol Plante Atmosphère (ISPA), Villenave d’Ornon, France;Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine, Bordeaux Sciences Agro, UMR1065 Santé et Agroécologie du Vignoble (SAVE), ISVV, Gradignan, France;INRAE, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Villenave d’Ornon, France;Université de Bordeaux, ISVV, UR Œnologie, Villenave d’Ornon, France;Université de Reims Champagne-Ardenne, Résistance Induite et Bioprotection des Plantes (RIBP), EA 4707 – USC INRAE 1488, Reims, France;

关键词: grapevine;    Esca;    gene expression;    sap flow;    stomatal activity;   

DOI  :  10.3389/fpls.2021.695846

来源: DOAJ

【 摘 要 】

Fungal species involved in Esca cause the formation of grapevine wood necroses. It results in the deterioration of vascular network transport capacity and the disturbance of the physiological processes, leading to gradual or sudden grapevine death. Herein, for two consecutive growing seasons, a detailed analysis of the structural (wood necrosis and leaf discoloration) and physiological parameters related to the water use of healthy and esca-symptomatic grapevines was conducted. Measurements were carried out on 17-year-old grapevines that expressed, or not, Esca-leaf symptoms in a vineyard of the Bordeaux region (France). Whole-plant transpiration was recorded continuously from pre-veraison to harvest, using noninvasive sap flow sensors. Whole-plant transpiration was systematically about 40–50% lower in Esca-diseased grapevines compared with controls, and this difference can be observed around 2 weeks before the first Esca-foliar symptoms appeared in the vineyard. Unlike grapevine sap flow disruption, structural (e.g., leaf discolorations), functional (e.g., stomatal conductance, photosynthetic activity, phenolic compounds), and genetic (e.g., expression of leaf-targeted genes) plant responses were only significantly impacted by Esca at the onset and during leaf symptoms development. We conclude that sap flow dynamic, which was related to a high level of a white-rot necrosis, provides a useful tool to predict plant disorders due to Esca-grapevine disease.

【 授权许可】

Unknown