First report of complete sequence of a blaNDM-13-harboring plasmid from an Escherichia coli ST5138 clinical isolate

Frontiers in Cellular and Infection Microbiology

Yiwei Tang¹  Liang Chen²  Barry Kreiswirth²  Fangyou Yu³  Xiuqing Qi³  Zengqiang Chen³  Shanshan Wang³  Yuehui Chen³  Dan Zhang³  Yunjian Guo³  Jinnzn Lv³  Zhou Zheng³  Liangxing Wang³  longhua Hu⁴ 

[1] Memorial Sloan Kettering Cancer Center;New Jersey Medical School, Rutgers University;The first Affiliated Hospital of Wenzhou Medical University;the Second Affiliated Hospital of Nanchang University;

关键词: Escherichia coli;    Genome;    Plasmid DNA;    carbapenemase;    NDM-13;   

DOI  :  10.3389/fcimb.2016.00130

来源: DOAJ

【 摘 要 】

Since the first report of blaNDM-1, 16 blaNDM variants have been identified among Gram-negative bacteria worldwide. Recently, a novel blaNDM variant, blaNDM-13, was identified in the chromosome of an ST101 Escherichia coli isolate from Nepal. Here we first reported plasmid-mediated blaNDM-13in a carbapenem-resistant E. coliST5138 clinical isolate associated with hospital-acquired urinary tract infection from China.blaNDM-13 andblaSHV-12coexisted on the a ~54 Kb self-transferable plasmid. Compared with NDM-1, NDM-13, NDM-3 and NDM-4 had two amino acid substitutions (D95N and M154L), one amino acid substitution (D95N) and one amino acid substitutions (M154L), respectively. Complete plasmid sequencing showed that blaNDM-13-harboring plasmid (pNDM13-DC33) was highly similar to the blaNDM-1–harboring IncX3 plasmid pNDM-HN380, a common blaNDM-harboring vector circulating in China. In accordance with the structure of pNDM-HN380, pNDM13-DC33 consists of a 33-kb backbone encoding plasmid replication (repB), stability partitioning, and transfer (tra, trb and pil) functions, and a 21-kb antimicrobial resistance region with high GC content between umuD and mpr genes. In conclusion, the present study is the first report of a plasmid-encoded blaNDM-13 and the complete sequence of a blaNDM-13-harboring plasmid (pNDM13-DC33). blaNDM-13 maybe originate from blaNDM-1 located on a pNDM-HN380-like plasmid by sequential mutations.

【 授权许可】

Unknown