Isolation and Characterization of Novel Lytic Bacteriophages Infecting Epidemic Carbapenem-Resistant Klebsiella pneumoniae Strains

Frontiers in Microbiology

Chaowang Zhu³  Min Guo⁴  Hong Du⁴  Long Chen⁵  Hongxiong Guo⁶  Liang Chen⁷  Wei Zhang⁹  Min Li⁹  Pei Li⁹  Yuyi Xiao⁹ 

[1] Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, United States;College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China;Department of Clinical Laboratory, The North District of Suzhou Municipal Hospital, Nanjing Medical University, Suzhou, China;Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, China;Department of Clinical Laboratory, Zhangjiagang Hospital Affiliated to Soochow University, Zhangjiagang, China;Department of Expanded Program on Immunization, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China;Hackensack Meridian School of Medicine, Seton Hall University, Nutley, NJ, United States;Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing, China;MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China;

关键词: bacteriophages;    carbapenem-resistant;    host range;    Klebsiella pneumoniae;    sewage;   

DOI  :  10.3389/fmicb.2020.01554

来源: DOAJ

【 摘 要 】

Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a significant clinical problem given the lack of therapeutic options available. Alternative antibacterial agents, such as bacteriophages, can be used as a valuable tool to treat the infections caused by these highly resistant bacteria. In this study, we isolated 54 phages from medical and domestic sewage wastewater between July and September 2019 and determined their host ranges against 54 clinical CRKP isolates, collected from a tertiary hospital in eastern China. The 54 CRKP isolates were from 7 sequence types (STs) and belonged to 9 capsular K locus types, harboring blaKPC–2 (n = 49), blaNDM–1 (n = 5), and blaIMP–4 (n = 3). Among them, the epidemic KPC-2-producing ST11 strains were most predominant (88.9%). The 54 phages showed different host ranges from 7 to 52 CRKP isolates. The total host ranges of three phages can potentially cover all 54 CRKP isolates. Among the 54 phages, phage P545, classified as a member of Myoviridaes, order Caudovirales, had a relatively wide host range (96.3%), a short latent period of 20 min, and a medium burst size of 82 PFU/cell and was stably maintained at different pH values (4–10) and temperatures (up to 60°C). P545 showed the ability to inhibit biofilm formation and to degrade the mature biofilms. Taken together, the results of our study showed that the newly isolated phage P545 had a relatively wide host range, excellent properties, and antibacterial activity as well as antibiofilm activity against a clinical CRKP ST11 isolate, providing a promising candidate for future phage therapy applications.

【 授权许可】

Unknown