Carboxy-terminal domain of Cas differentially modulates c-Jun expression, DNA synthesis, and membrane ruffling induced by insulin, EGF, and IGF-1

Animal Cells and Systems

Byung H. Jhun¹  Myung-ju Oh¹  Kyunghwan Kim²  Seong Yun Hwang²  Sun-Ju Yi² 

[1] Pusan National University;School of Biological Sciences, College of Natural Sciences, Chungbuk National University;

关键词: Cas;    microinjection;    EGF;    insulin;    IGF-1;    c-Jun expression;    DNA synthesis;    membrane ruffling;   

DOI  :  10.1080/19768354.2018.1447013

来源: DOAJ

【 摘 要 】

p130 Crk-associated substrate (Cas) is an adaptor protein associating with many other signaling proteins and regulates a various biological processes including cell adhesion, migration, and growth factor stimulation. However, the exact functional role of Cas in growth factor signaling pathway was poorly understood. Here we investigated the role of Cas and its domains in the effects of insulin, EGF, and IGF-1 on c-Jun gene expression, DNA synthesis, cytoskeletal reorganization. We found that microinjection of anti-Cas antibody and C-terminal domain of Cas (Cas-CT) specifically inhibited EGF-induced, but not insulin- or IGF-1-induced, c-Jun expression. Cell cycle progression and cytoskeleton reorganization induced by insulin and EGF, but not by IGF-1, were inhibited by microinjected anti-Cas and Cas-CT. In contrast, microinjection of the substate domain (Cas-SD) of Cas did not have any inhibitory effects. These results revealed that the Cas-CT is differentially implicated in insulin and EGF-mediated, but not IGF-1-mediated, c-Jun expression, DNA synthesis and membrane ruffling.

【 授权许可】

Unknown