In vivo and in vitro evidence of basic fibroblast growth factor action in mouse mammary gland development

FEBS Letters

Lavandero, Sergio¹  Chappuzeau, Andrés¹  Oka, Takami²  Sapag-Hagar, Mario¹ 

[1] Departamento de Bioquı́mica y Biologı́a Molecular, Olivos 1007, Facultad de Ciencias Quı́micas y Farmacéuticas, Universidad de Chile, P.O. Box 233, Santiago, Chile;Laboratory of Genetics and Physiology, Building 8, Room 106, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Basic fibroblast growth factor;    Growth factor;    Milk protein;    Mammary gland;    Gene expression;    bFGF;    basic fibroblast growth factor;    EGF;    epidermal growth factor;    F;    hydrocortisone;    I;    insulin;    MMEC;    mouse mammary epithelial cells;    P;    prolactin;   

DOI  :  10.1016/S0014-5793(98)01370-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Basic fibroblast growth factor (bFGF) stimulated [³H]thymidine incorporation at all stages of development, although the magnitude of this effect was the greatest in cells derived from pregnant mice. Cells primed with insulin and bFGF synthesized more casein than cells not exposed to either hormone. bFGF inhibited casein synthesis and decreased the amounts of β-casein and α-lactalbumin transcripts in cells from pregnant animals simultaneously incubated with insulin, hydrocortisone and prolactin. bFGF content in mammary gland increased with puberty and pregnancy, but decreased markedly in lactation; the number of bFGF receptors in epithelial cells changed in parallel. These data suggest that bFGF may have a physiological role both in stimulating growth and in inhibiting functional differentiation of normal mouse mammary epithelial cells.

【 授权许可】

Unknown