Dihydrocapsaicin inhibits cell transformation in JB6P+ mouse epidermal cells by regulating mTOR pathway and c-fos

Dihydrocapsaicin;mTOR pathway;EGF;AP1(activator protein 1);571

농업생명과학대학 농생명공학부(바이오모듈레이션전공) ;

University:서울대학교 대학원

关键词: Dihydrocapsaicin;    mTOR pathway;    EGF;    AP1(activator protein 1);    571;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/126045/1/000000012879.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

Chili pepper has been reported to possess anti-carcinogenic effects. But there are articles about carcinogenic effect of chili pepper. Capsaicin which is a main component in chili pepper has a controversial point whether it has cancer preventive or promotive effect. Anti-carcinogenic effects of chili pepper components except capsaicin, for example, dihydrocapsaicin, capsiate, and capsanthin, are still unclear. Herein, I examined the inhibition effect of neoplastic transformation on chili pepper components and associated mechanisms of dihydrocapsaicin in JB6 P+ cell line. Epidermal growth factor (EGF)-induced neoplastic transformation was inhibited by 50 M dihydrocapsaicin in JB6 P+ cells. Dihydrocapsaicin suppressed EGF-induced cyclooxygenase-2 (COX-2) promoter activity and activator protein-1 (AP-1) and nuclear factor-κB (NF-κB) transactivations in JB6 P+ cells. Immunoblot data showed that dihydrocapsaicin inhibited EGF-induced phosphorylation of mTOR signaling pathway and expression of c-fos. Together, these findings suggest that dihydrocapsaicin exerts potent inhibitor of neoplastic transformation against EGF-induced skin inflammation.