MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration

Bioactive Materials

Dandan Ma¹  Wenjing Song²  He Wang³  Haiyue Yu⁴  Yingjun Wang⁵  Xiaodong Cao⁶  Hongbao Xin⁷  Ting Pan⁷ 

[1] Corresponding author. School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510006, PR China.;Institute of Nanophotonics, Jinan University, Guangzhou, 511443, PR China;Key Laboratory of Biomedical Engineering of Guangdong Province, And Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, China;National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, China;Charité–Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Department of Oral Diagnosis, Digital Health and Health Services Research, Berlin, Germany;Institute of Nanophotonics, Jinan University, Guangzhou, 511443, PR China;School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510006, PR China;

关键词: Bone repair;    microRNA therapy;    Scaffold;    Osteogenesis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Bone defects remain a major threat to human health and bone tissue regeneration has become a prominent clinical demand worldwide. The combination of microRNA (miRNA) therapy with 3D printed scaffolds has always posed a challenge. It can mimic physiological bone healing processes, in which a biodegradable scaffold is gradually replaced by neo-tissue, and the sustained release of miRNA plays a vital role in creating an optimal osteogenic microenvironment, thus achieving promising bone repair outcomes. However, the balance between two key factors - scaffold degradation behavior and miRNA release profile - on osteogenesis and bone formation is still poorly understood. Herein, we construct a series of miRNA-activated hydrogel scaffolds (MAHSs) generated by 3D printing with different crosslinking degree to screened the interplay between scaffold degradation and miRNA release in the osteoinduction activity both in vitro and in vivo. Although MAHSs with a lower crosslinking degree (MAHS-0 and MAHS-0.25) released a higher amount of miR-29b in a sustained release profile, they degraded too fast to provide prolonged support for cell and tissue ingrowth. On the contrary, although the slow degradation of MAHSs with a higher crosslinking degree (MAHS-1 and MAHS-2.5) led to insufficient release of miR-29b, their adaptable degradation rate endowed them with more efficient osteoinductive behavior over the long term. MAHS-1 gave the most well-matched degradation rate and miR-29b release characteristics and was identified as the preferred MAHSs for accelerated bone regeneration. This study suggests that the bio-adaptable balance between scaffold degradation behavior and bioactive factors release profile plays a critical role in bone regeneration. These findings will provide a valuable reference about designing miRNAs as well as other bioactive molecules activated scaffold for tissue regeneration.

【 授权许可】

Unknown