Infection by and genotype characteristics of Enterocytozoon bieneusi in HIV/AIDS patients from Guangxi Zhuang autonomous region, China

BMC Infectious Diseases

Zunfu Wang¹  Dongsheng Zhou¹  Zhihua Jiang¹  Yujuan Shen²  Jianping Cao²  Zhongying Yuan³  Jianhai Yin⁴  Hua Liu⁴  Bingxue Yu⁴ 

[1] Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center for Tropical Diseases;Guangxi Medical University;Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention;National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention;

关键词: Enterocytozoon bieneusi;    HIV/AIDS;    Genotype;    Risk factors;   

DOI  :  10.1186/s12879-017-2787-9

来源: DOAJ

【 摘 要 】

Abstract Background Enterocytozoon bieneusi has been increasingly reported to infect humans and various mammals. Microsporidia cause diarrhea in HIV-infected patients worldwide. PCR amplification and sequencing based on the internal transcribed spacer region have been used to describe the genotypes of E. bieneusi and transmission of microsporidiosis. Methods In this study, we examined E. bieneusi infection and genotypes in HIV-positive patients in Guangxi, China. Stool specimens were collected from 285 HIV-positive patients and 303 HIV-negative individuals. E. bieneusi genotypes were characterized using nested PCR and sequencing. Results Thirty-three (11.58%) HIV-positive patients were infected with microsporidia, and no infection was found in the 303 healthy controls. Three new genotypes were identified and named as GX25, GX456, and GX458; four known genotypes, PigEBITS7, Type IV/K, D, and Ebpc, were also identified. Our data showed that the positive rate for microsporidia was significantly higher in the rural patients than in the other occupation groups. In addition, the positive rate for microsporidia was significantly higher in the patients who drink unboiled water than in those with other drinking water sources. Conclusions Our results will provide baseline data for preventing and controlling E. bieneusi infection in HIV/AIDS patients. Further studies are required to clarify the epidemiology and potential sources of microsporidia. Our study showed that microsporidium infection occurs in the HIV/AIDS patients in Guangxi, China.

【 授权许可】

Unknown