Population genetic analysis suggests genetic recombination is responsible for increased zoonotic potential of Enterocytozoon bieneusi from ruminants in China

One Health

Jingbo Ma¹  Zhenjie Zhang²  Xi Wen²  Yaqiong Guo²  Yaoyu Feng²  Longxian Zhang²  Wen Jiang²  Lihua Xiao³  Xitong Huang⁴  Li Chen⁵  Na Li⁵ 

[1] Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, Guangdong 510642, China;Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China;College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;Department of Parasitology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China;School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;

关键词: Enterocytozoon bieneusi;    Genotype;    Population genetics;    China;    Zoonotic infection;   

DOI  :  

来源: DOAJ

【 摘 要 】

Enterocytozoon bieneusi is a zoonotic pathogen with worldwide distribution. Among the 11 established groups of E. bieneusi genotypes based on phylogenetic analysis of the ribosomal internal transcribed spacer (ITS), the human-infective potential and population genetics of the Group 1 genotypes from diverse hosts are well characterized. In contrast, Group 2 genotypes from ruminants have unclear population genetics, leading to poor understanding of their host range and zoonotic potential. In this study, we sequence-characterized 121 Group 2 isolates from dairy cattle, beef cattle, yaks, Tibetan sheep, golden takins, and deer from China at five genetic loci (ITS, MS1, MS3, MS4 and MS7), comparing with data from 113 Group 1 isolates from nonhuman primates. Except for MS7, most of the genetic loci produced efficient PCR amplification and high nucleotide identity between Groups 1 and 2 of E. bieneusi genotypes. In population genetic analyses of the sequence data, a strong linkage disequilibrium was observed among these genetic loci in the overall Group 2 population. The individual ITS genotypes (I, J and BEB4) within Group 2, however, had reduced linkage disequilibrium and increased genetic exchanges among isolates. There was only partial genetic differentiation between Group 1 and Group 2 genotypes, with some occurrence of genetic recombination between them. Genetic recombination was especially common between genotypes I and J within Group 2. The data presented indicate a high genetic identity between Group 1 and Group 2 genotypes of E. bieneusi, which could be responsible for the broad host range and high zoonotic potential of Group 2 genotypes in China. As there is no effective treatment against E. bieneusi, the One Health approach should be used in the control and prevention of zoonotic transmission of the pathogen.

【 授权许可】

Unknown